Human FcRn / FCGRT&B2M Heterodimer Protein, His & Strep II Tag (SPR & BLI verified)

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
FcRn,FCGRT & B2M
Source
Human FCGRT&B2M Heterodimer Protein, His&Strep II Tag (SPR & BLI verified) (FCM-H5286) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # NP_001129491.1 (FCGRT) & AAH32589 (B2M)).
Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)
Request for sequence
Molecular Characterization
Human FCGRT&B2M Heterodimer Protein, His&Strep II Tag (SPR & BLI verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 31.2 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human FCGRT&B2M Heterodimer Protein, His&Strep II Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR

Immobilized Human FCGRT&B2M Heterodimer Protein, His&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H5286) on CM5 Chip via Anti-His antibody, can bind Herceptin with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200) (QC tested).

Request for protocol

Immobilized Human FCGRT&B2M Heterodimer Protein, His&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H5286) on CM5 Chip, can bind Herceptin with an affinity constant of 1.19 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Request for protocol

Immobilized Herceptin on CM5 Chip can bind Human FCGRT&B2M Heterodimer Protein, His&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H5286) with an affinity constant of 0.998 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Request for protocol

Bioactivity-BLI

Loaded Human FCGRT&B2M Heterodimer Protein, His&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H5286) on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.27 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Request for protocol