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Your Position: Home > Viral Proteins > Glycoprotein B / gB > CMB-V52H5

HCMV (strain Merlin) Glycoprotein B (gB) (I156H, H157R, W240N, Y242T, C246S, R457S, R460S), His Tag (MALS verified)

  • Synonym
    Glycoprotein B,gB,Envelope glycoprotein B
  • Source
    HCMV (strain Merlin) Glycoprotein B (gB), His Tag (CMB-V52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 25 - Glu 698 (Accession # F5HB53-1(I156H, H157R, W240N, Y242T, C246S, R457S, R460S)).
    Predicted N-terminus: Ser 25
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 78.7 kDa. The protein migrates as 105-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HCMV (strain Merlin) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H5) SDS-PAGE gel

HCMV (strain Merlin) Glycoprotein B (gB), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
HCMV (strain Merlin) Glycoprotein B (gB), His Tag (Cat. No. ) MALS images

The purity of HCMV (strain Merlin) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H5) is more than 95% and the molecular weight of this protein is around 315-386 kDa verified by SEC-MALS.

Bioactivity-ELISA
HCMV (strain Merlin) Glycoprotein B (gB), His TagHCMV (strain Merlin) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H5) ELISA bioactivity

Immobilized HCMV (strain Merlin) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H5) at 1 μg/mL (100 μL/well) can bind Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 (Cat. No. GLB-M330) with a linear range of 0.2-8 ng/mL (QC tested).

HCMV (strain Merlin) Glycoprotein B (gB), His TagHCMV (strain Merlin) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H5) ELISA bioactivity

Immobilized Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 (Cat. No. GLB-M330) at 1 μg/mL (100 μL/well) can bind HCMV (strain Merlin) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H) with a linear range of 1-39 ng/mL (Routinely tested).

  • Background
    Human cytomegalovirus is a species of the Cytomegalovirus genus of viruses, which in turn is a member of the viral family known as Herpesviridae or herpesviruses. It is typically abbreviated as HCMV or, commonly but more ambiguously, as CMV. CMV Virus Envelope Glycoportein B (CMV-GB) can be cleaved into glycoprotein GP55. Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. CMV-GB may be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Furthermore, CMV-GB can interact with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Also, CMV-GB participates in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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