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Your Position: Home > Immune Checkpoint Proteins > PD-1 > Human PDCD1 PD1 Protein, With C-Fc Tag

Human PD-1 / PDCD1 Protein, Fc Tag (HPLC-verified), low endotoxin

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    Human PD-1, Fc Tag (PD1-H5257) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_005009.2).
    N-terminal Sequence Analysis: Leu 25
  • Molecular Characterization
    Online(Leu 25 - Gln 167) NP_005009.2

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 42.6 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human PD-1, Fc Tag (Cat. No. PD1-H5257) SDS-PAGE gel

Human PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Human PD-1, Fc Tag (Cat. No. ) HPLC images

The purity of Human PD-1, Fc Tag (HPLC-verified)(Cat. No. PD1-H5257) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
Human PD-1, Fc TagHuman PD-1, Fc Tag (Cat. No. PD1-H5257) ELISA bioactivity

Immobilized Biotinylated Human PD-L1 (recommended for biopanning), His Tag, Avi Tag (Cat. No. PD1-H82E5) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2μg/well) plate, can bind Human PD-1, Fc Tag (Cat. No. PD1-H5257) with a linear range of 0.1-3 ng/mL (QC tested).

Human PD-1, Fc TagHuman PD-1, Fc Tag (Cat. No. PD1-H5257) ELISA bioactivity

Immobilized Human PD-1, Fc Tag (Cat. No. PD1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L2, Fc Tag, Avi Tag (Cat. No. PD2-H82F6) with a linear range of 0.5-16 ng/mL. (Routinely tested).

Human PD-1, Fc TagHuman PD-1, Fc Tag (Cat. No. PD1-H5257) ELISA bioactivity

Immobilized Biotinylated Human PD-L2, His Tag (Cat. No. PD2-H82E8) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2μg/well) plate, can bind Human PD-1, Fc Tag (Cat. No. PD1-H5257) with a linear range of 0.1-1 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human PD-1, Fc Tag (Cat. No. PD1-H5257) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (HPLC-verified) (Cat. No. PD1-H5229) with an affinity constant of 3.6 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

Flow Cytometry assay shows that recombinant Human PD-1, Fc Tag (Cat. No. PD1-H5257) can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 μg/mL (Routinely tested).

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