Login Register
icon_bulk_orderBulk order Acrobiosystems for English
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Non SARS-CoV-2 Beads > CD3 & CD28 > MBS-C001

Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed

Materials Provided
ProductSizeAmount
Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed2.5 mg2.5 × 107 beads
10 mg1 × 108 beads
  • Background
    Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed are uniform 5.5 μm of magnetic beads coated with an optimized mixture of monoclonal antibodies against the CD3 and CD28. The CD3 antibody is specific for the epsilon chain of human CD3, which is considered to be a subunit of the TCR complex. The CD28 antibody is specific for the human CD28 co-stimulatory molecule, which is the receptor for CD80 (B7-1) and CD86 (B7-2). Both antibodies are mouse anti-human IgGs coupled to the same bead, mimicking in vivo stimulation by APCs.
  • Application
    Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed is intended for the in vitro stimulation and expansion of purified T cell populations of, for example, CD3+T cells, CD4+ T cells, CD8+ T cells or human PBMC.
    This is for research use and ex vivo cell culture processing only, and is not intended for human in vivo applications.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Endotoxin
    Less than 2EU/mg by the LAL method.
  • Reconstitution
    See Certificate of Analysis (CoA) for detailed instruction.
  • Storage

    This product is stable after storage at: -20˚C for 24 months in lyophilized state; 2˚C to 8˚C for 24 months under sterile conditions after reconstitution.

    Please avoid freezing after reconstitution. Immediate use after reconstitution is highly recommended.

  • Important Note
    This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
  • Data

    Fig.1.  Activation of the purified human T Cells. The purified human T cells were activated using Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed (Cat. No. MBS-C001) at a ratio of 1:1 beads-to-cells for 24 hours with RPMI1640 supplemented with 10% of FBS. The negative control experiment was performed with adding the Negative Control Beads coupled HSA. Cells were fluorescently stained using PE labeled anti-human CD25 antibody and labeled FITC anti-human CD69 antibody and analyzed by flow cytometry.


    Fig.2.  Purified human T Cells expansion. The purified human T cells were stimulated using Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed (Cat. No. MBS-C001) at a ratio of 1:1 beads-to-cells. Cells were expanded in T cell culture medium supplemented with 4ng/mL of rhIL-2 Protein (Acrobiosystems, Cat. No. IL2-H4113). Activated Cells were expanded for up to 13 days (A) with high cell viability (B).

    Fig.3.  PD-1 expression of the activated human T Cells. The purified human T cells were stimulated using Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed (Cat. No. MBS-C001) at a ratio of 1:1 beads-to-cells. Cells were expanded in T cell culture medium supplemented with 4ng/mL of rhIL-2 Protein (Acrobiosystems, Cat. No. IL2-H4113). Activated T cells were expanded for up to 10 days with low PD-1 expression.

    Fig.4 Activation of the purified human T Cells with IL-2 in different medium. The purified human T cells were stimulated using Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed (Cat. No. MBS-C001) at a ratio of 1:1 beads-to-cells in three commercial medium, Human rh IL-2 (Cat. No. IL2-H5215,  4ng/mL) was added. The expansion ability of T cells varied greatly in the culture medium from different manufacturers.

Comments (0)


ETD of in-stock products: 2 business days

Price(USD) : $200.00

Price(USD) : $550.00

Price(USD) : Inquire

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America: +1 800-810-0816 (Toll Free)
Asia & Pacific: +86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message