|Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed||2.5 mg||2.5 × 107 beads|
|10 mg||1 × 108 beads|
This product is stable after storage at: -20˚C for 24 months in lyophilized state; 2˚C to 8˚C for 24 months under sterile conditions after reconstitution.
Please avoid freezing after reconstitution. Immediate use after reconstitution is highly recommended.
Fig.1. Activation of the purified human T Cells. The purified human T cells were activated using Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed (Cat. No. MBS-C001) at a ratio of 1:1 beads-to-cells for 24 hours with RPMI1640 supplemented with 10% of FBS. The negative control experiment was performed with adding the Negative Control Beads coupled HSA. Cells were fluorescently stained using PE labeled anti-human CD25 antibody and labeled FITC anti-human CD69 antibody and analyzed by flow cytometry.
Fig.2. Purified human T Cells expansion. The purified human T cells were stimulated using Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed (Cat. No. MBS-C001) at a ratio of 1:1 beads-to-cells. Cells were expanded in T cell culture medium supplemented with 4ng/mL of rhIL-2 Protein (Acrobiosystems, Cat. No. IL2-H4113). Activated Cells were expanded for up to 13 days (A) with high cell viability (B).
Fig.3. PD-1 expression of the activated human T Cells. The purified human T cells were stimulated using Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed (Cat. No. MBS-C001) at a ratio of 1:1 beads-to-cells. Cells were expanded in T cell culture medium supplemented with 4ng/mL of rhIL-2 Protein (Acrobiosystems, Cat. No. IL2-H4113). Activated T cells were expanded for up to 10 days with low PD-1 expression.
Fig.4 Activation of the purified human T Cells with IL-2 in different medium. The purified human T cells were stimulated using Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed (Cat. No. MBS-C001) at a ratio of 1:1 beads-to-cells in three commercial medium, Human rh IL-2 (Cat. No. IL2-H5215, 4ng/mL) was added. The expansion ability of T cells varied greatly in the culture medium from different manufacturers.
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