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Your Position: Home > Fc Receptor Proteins > HLA-G > HLG-H52E9

Human HLA-G RIIPRHLQL Tetramer Protein (MALS verified)

  • Synonym
    HLA-G, HLA class I histocompatibility antigen, alpha chain G, HLAG, HLA-6.0
  • Source
    Human HLA-G RIIPRHLQL Tetramer Protein (HLG-H52E9) is expressed from human 293 cells (HEK293). It contains AA Gly 25 -  Ile 308 (HLA-G) & Ile 21 - Met 119 (B2M) & RIIPRHLQL peptide (Accession # P17693-1(HLA-G) & P61769-1(B2M)).
    Predicted N-terminus: Gly 25 (HLA-G) & Arg (B2M)
  • Molecular Characterization

    Human HLA-G Complex Tetramer Protein is assembled by biotinylated monomer and streptavidin. The biotinylated monomer is assembled by co-expression of histone H2A covalently linked with B2M and HLA. The biotinylated Human HLA-G Complex monomer carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The calculated MW of HLA, B2M and streptavidin are 36.3 kDa, 13.9 and 13.3 kDa, respectively. The protein migrates as 42-45 kDa and 15 kDa respectively under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human HLA-G RIIPRHLQL Tetramer Protein (Cat. No. HLG-H52E9) SDS-PAGE gel

Human HLA-G RIIPRHLQL Tetramer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human HLA-G RIIPRHLQL Tetramer Protein (Cat. No. ) MALS images

The purity of Human HLA-G RIIPRHLQL Tetramer Protein (Cat. No. HLG-H52E9) is more than 85% and the molecular weight of this protein is around 228-278 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human HLA-G RIIPRHLQL Tetramer ProteinHuman HLA-G RIIPRHLQL Tetramer Protein (Cat. No. HLG-H52E9) ELISA bioactivity

Serial dilutions of Anti-LILRB2 Antibody, Human IgG1 were added into Human LILRB2, Fc Tag (Cat. No. CDD-H5259) : Human HLA-G RIIPRHLQL Tetramer Protein (Cat. No. HLG-H52E9) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1486  μg/mL (QC tested).

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