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This protein carries a human IgG1 Fc tag at the N-terminus
The protein has a calculated MW of 94.0 kDa & 35.0 kDa & 35.7 kDa. The protein migrates as 70-80 kDa and 116-125 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 51 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl,pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Laminin 111 E8, Fc Tag, premium grade on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human Laminin 111 E8 Protein, Fc Tag, premium grade (Cat. No. LA1-H5269) at 5 μg/mL (100 μL/well) can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) at 5 μg/mL (100 μL/well) can bind Human Laminin 111 E8 Protein, Fc Tag, premium grade (Cat. No. LA1-H5269) with a linear range of 0.5-31 ng/mL (Routinely tested).
Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) at 5 μg/mL (100 μL/well) can bind Human Laminin 111 E8 Protein, Fc Tag, premium grade (Cat. No. LA1-H5269) with a linear range of 0.2-2 ng/mL (Routinely tested).
Loaded Human Laminin 111 E8, Fc Tag, premium grade (Cat. No. LA1-H5269) on Protein A Biosensor, can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with an affinity constant of 12.4 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) on NTA Biosensor, can bind Human Laminin 111 E8 Protein, Fc Tag, premium grade (Cat. No. LA1-H5269) with an affinity constant of 5.33 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $120.00
Price(USD) : $650.00
ACROBiosystems offers a wide selection of Matrigengel basement membrane extracts. Different versions – including phenol red-free, high concentration, iPSC culturing, organoid culturing – are all available for your specific needs to support 3D cell culture!
To enable antibody characterization methodsACROBiosystems has developed a series of enzymes.such as ldeS, SpeB, EndoH, and Endo S proteases, toassist with the characterization of antibodies and theirrelated post-translational modifications (PTMs)
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