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Your Position: Home > Non SARS-Cov-2 Kits > Glycoprotein E (VZV) > RAS-T103

Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE)

For research use only.
Materials Provided
IDComponentsSize
RAS103-C01Pre-coated Glycoprotein E (VZV) Microplate1 plate
RAS103-C02Positive Control100 μL
RAS103-C03Negative Control100 μL
RAS103-C04HRP-Goat anti-Mouse IgG 50 μL
RAS103-C0510 x Washing Buffer 50 mL
RAS103-C06Dilution Buffer50 mL
RAS103-C07Substrate Solution12 mL
RAS103-C08Stop Solution7 mL
  • Background
    Varicella-zoster virus (VZV) the etiologic agent of chickenpox and herpes zoster [HZ], is highly contagious and still endemic worldwide. Glycoprotein E (gE) is one of the known glycoproteins (gB, gC, gE, gH, gI, gK, gI) of VZV that is most abundantly expressed on the surface of virus and infected cells, playing an important role in viral replication and cell-to-cell spread. The strongly immunogenic gE can provide strong IgG signal in body fluid, which makes it ideal to be developed as an antigen for analysis of Immunogenicity in the development of VZV vaccine.Therefore,It's helpful to develop the Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE) to detection the IgG(GE) titer level in mouse serum during the preclinical stage of vaccine development.
  • Application
    This kit is developed for titer detection of Anti-Varicella Zoster Virus Antibody IgG (gE) in mouse serum.
    It is for research use only.
  • Storage

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-VZV gE antibodies in Mouse serum by Glycoprotein E (VZV). The Kit consists of Pre-coated Glycoprotein E (VZV) Microplate, an Positive Control, an Negative Control, an HRP-Anti-Goat anti mouse IgG secondary antibody.

    Your experiment will include 4 simple steps:

    a) The samples and Control are diluted by Dilution Buffer. Add your sample to the plate.

    b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE)Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE) (Cat. No. RAS-T103) ELISA bioactivity

For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.

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ETD of in-stock products: 2 business days

Price(USD) : $580.00

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