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Your Position: Home > Non SARS-Cov-2 Kits > Bevacizumab > RAS-T122

Human Bevacizumab Quantitative Detection ELISA Kit

For research use only.
IDComponentsSize
RAS122-C01Pre-coated Anti-Bevacizumab Antibody Microplate1 plate
RAS122-C02Bevacizumab Standard100 μL
RAS122-C03Biotin-Anti-Bevacizumab Antibody100 μL
RAS122-C04Streptavidin-HRP50 μL
RAS122-C0510xWashing Buffer 50 mL
RAS122-C062xDilution Buffer50 mL
RAS122-C07Substrate Solution12 mL
RAS122-C08Stop Solution7 mL
  • Background
    A recombinant humanized monoclonal IgG1 antibody that binds to and inhibits the biologic activity of human vascular endothelial growth factor (VEGF). Bevacizumab contains human framework regions and the complementarity-determining regions of a murine antibody that binds to VEGF. Bevacizumab is produced in a Chinese Hamster Ovary mammalian cell expression system in a nutrient medium containing the antibiotic gentamicin and has a molecular weight of approximately 149 kilodaltons.
  • Application
    The kit is developed for quantitative detection of Bevacizumab antibody in human serum.

    It is for research use only.

  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Bevacizumab. The kit consists of Pre-coated Anti-Bevacizumab Antibody Microplate and Bevacizumab Standard and Biotin-Anti-Bevacizumab Antibody and Streptavidin-HRP and buffers.

    Your experiment will include 6 simple steps:

    a) All reagents were returned to room temperature(20°C-25°C) before use.

    b) Add your sample to the plate, take the Bevacizumab as standard sample. The samples and standard sample are diluted by Dilution Buffer.

    c) Wash the plate and add a diluted Biotin-Anti-Bevacizumab Antibody to the plate. The diluted by Dilution Buffer.

    d) Wash the plate and add a diluted Streptavidin-HRP to the plate. The diluted by Dilution Buffer.

    e) Wash the plate and add TMB.

    f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data
 Bevacizumab ELISA

For each experiment, each ELISA plate needs to set the standard curve. The following RAS-T122 standard curve data is for reference only.

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Price(USD) : $1200.00

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