|RAS122-C01||Pre-coated Anti-Bevacizumab Antibody Microplate||1 plate|
|RAS122-C02||Bevacizumab Standard||100 μL|
|RAS122-C03||Biotin-Anti-Bevacizumab Antibody||100 μL|
|RAS122-C05||10xWashing Buffer||50 mL|
|RAS122-C06||2xDilution Buffer||50 mL|
|RAS122-C07||Substrate Solution||12 mL|
|RAS122-C08||Stop Solution||7 mL|
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 6 simple steps:
a) All reagents were returned to room temperature(20°C-25°C) before use.
b) Add your sample to the plate, take the Bevacizumab as standard sample. The samples and standard sample are diluted by Dilution Buffer.
c) Wash the plate and add a diluted Biotin-Anti-Bevacizumab Antibody to the plate. The diluted by Dilution Buffer.
d) Wash the plate and add a diluted Streptavidin-HRP to the plate. The diluted by Dilution Buffer.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
For each experiment, each ELISA plate needs to set the standard curve. The following RAS-T122 standard curve data is for reference only.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $1200.00
ComboX™ is ACROBiosystems new brand. ComboX™ products include our Streptavidin series products, coated plates, isotype controls for antibody and fusion protein drug research, and general-purpose antibodies.
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