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Your Position: Home > Kits > Spike RBD > RAS-A130

SARS-CoV-2 Spike RBD (BA.2.12.1) ELISA Kit

For research use only.

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IDComponentsSize
RAS130-C01Pre-coated Anti-SARS-CoV-2 Spike RBD Antibody Microplate1 plate
RAS130-C02SARS-CoV-2 Spike RBD (BA.2.12.1)15 μg
RAS130-C03HRP-Anti-SARS-CoV-2 Spike RBD Antibody30 μg
RAS130-C0410xWashing Buffer 50 mL
RAS130-C05Dilution Buffer50 mL
RAS130-C06Substrate Solution12 mL
RAS130-C07Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike protein is urgently needed to accelerate the development of COVID-19 vaccines.
  • Application

    This kit is developed for quantitative detection of SARS-CoV-2 Spike RBD (BA.2.12.1) in samples.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage & Shipping
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Shipping Statement

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike RBD. The kit consists of microplate pre-coated Anti-SARS-CoV-2 Spike RBD Antibody, an SARS-CoV-2 Spike RBD (BA.2.12.1) as Control, an biotin-Anti-SARS-CoV-2 Spike RBD Antibody, HRP-Streptavidin and buffers.

    Your experiment will include 5 simple steps:

    a) All reagents were returned to room temperature(20°C-25°C) before use.

    b) Add your sample to the plate, take the SARS-CoV-2 Spike RBD as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add a diluted HRP-Anti-SARS-CoV-2 Spike RBD Antibody to the plate. The diluted by Dilution Buffer.

    d) Wash the plate and add TMB.

    e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data Please refer to DS document for The assay protocol.
 Spike RBD TYPICAL DATA

Detection of SARS-CoV-2 Spike RBD (BA.2.12.1) by sandwich-ELISA Assay.
Immobilized Anti-SARS-CoV-2 Spike RBD Antibody can bind SARS-CoV-2 Spike RBD (BA.2.12.1). Detection was performed using HRP-Anti-SARS-CoV-2 Spike RBD Antibody with sensitivity of 0.39 ng/mL (QC tested).

  • Clinical and Translational Updates

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Price(USD) : $780.00

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