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Your Position: Home > Non SARS-CoV-2 Beads > Protein G > MPC-A002

Protein G-coupled Magnetic Beads (used for MPCLIA)

Materials Provided
ItemsDetails
Detection MethodChemiluminescence
Product TypeMagnetic Beads (Protein G)
Quantity Size10mg / 100mg
Physical Appearancelyophilized powder mixture
Particle size2.8µm
Beads SurfaceHydrophilic
Amount of Coupled ProteinAbout 400 pmol (9 μg) Protein G/mg Beads
Binding Capacity5-10ug antibody or Human IgG Fc tag proteins/mg beads
Emission WavelengthMeasured relative light units (RLU) at 430 nm
  • Background
    The Protein G-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of protein G. The beads can be used to capture the antibodies in Chemiluminescence procedures. The Protein G binds the heavy chain within the Fc region of most immunoglobulins but not bind dog immunoglobulins. The Protein G-coupled Magnetic Beads is easy to capture the most antibodies, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
  • Application
    The Protein G-coupled Magnetic Beads is used to capture the most antibodies, it can combination with Acridine ester markers in chemiluminescence technology, The Acridine ester markers such as Streptavidin-Acridine Ester can capture the biotinylated proteins or molecules, this allows detection of antigen and antibody binding or antibody screening.
  • Reconstitution

    See Certificate of Analysis (CoA) for detailed instruction.

  • Storage
    Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
    Bioactivity-MPCLIA
     Protein G MPCLIA

    Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL/Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Protein G-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A002) (10 μg beads/Test). Detection was performed with sensitivity of 3.9 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

     Protein G MPCLIA

    The MPCLIA assay shows that Protein G-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A002) is stable at 37°C for 10 days.

     Protein G MPCLIA

    The MPCLIA assay shows that Protein G-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A002) is stable after freezing and thawing 3 times.

     Protein G MPCLIA

    The MPCLIA assay shows that Protein G-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A002) is stable at 4°C for 30 days.

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    ETD of in-stock products: 2 business days

    Price(USD) : $88.00

    Price(USD) : $800.00

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