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| Items | Details |
| Detection Method | Chemiluminescence |
| Product Type | Magnetic Beads (Protein A) |
| Quantity Size | 10mg / 100mg |
| Physical Appearance | lyophilized powder mixture |
| Particle size | 2.8µm |
| Beads Surface | Hydrophilic |
| Amount of Coupled Protein | About 755 pmol (20 μg) Protein A/mg Beads |
| Binding Capacity | 15-25ug Human antibody or Human IgG Fc tag proteins/mg beads |
| Emission Wavelength | Measured relative light units (RLU) at 430 nm |
See Certificate of Analysis (CoA) for detailed instruction.
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Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Protein A-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A001) (10 μg beads/Test). Detection was performed with sensitivity of 3.125 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
The MPCLIA assay shows that Protein A-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A001) is stable at 37°C for 10 days.
The MPCLIA assay shows that Protein A-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A001) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Protein A-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A001) is stable at 37°C for 3 days.
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