This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 13.5 kDa. The protein migrates as 16-17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Vibrio cholerae serotype O1 Cholera enterotoxin subunit B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Vibrio cholerae serotype O1 Cholera enterotoxin subunit B, His Tag (Cat. No. GHB-V52H3) is more than 85% and the molecular weight of this protein is around 73-83kDa verified by SEC-MALS.
Immobilized Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) Cholera enterotoxin subunit B, His Tag (Cat. No. GHB-V52H3) at 1 μg/mL (100 μL/well) can bind Anti-Cholera toxin, B Subunit (CTxB) Antibody with a linear range of 0.1-2 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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