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Your Position: Home > Virus > Cholera enterotoxin subunit B > GHB-V52H3

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) Cholera enterotoxin subunit B Protein, His Tag (MALS verified)

  • Synonym
    CE subunit B
  • Source
    Vibrio cholerae serotype O1 Cholera enterotoxin subunit B, His Tag(GHB-V52H3) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Asn 124 (Accession # P01556 ).
    Predicted N-terminus: Thr 22
  • Molecular Characterization
    Cholera enterotoxin subunit B Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 13.5 kDa. The protein migrates as 16-17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cholera enterotoxin subunit B SDS-PAGE

Vibrio cholerae serotype O1 Cholera enterotoxin subunit B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Cholera enterotoxin subunit B MALS images

The purity of Vibrio cholerae serotype O1 Cholera enterotoxin subunit B, His Tag (Cat. No. GHB-V52H3) is more than 85% and the molecular weight of this protein is around 73-83kDa verified by SEC-MALS.

Bioactivity-ELISA
 Cholera enterotoxin subunit B ELISA

Immobilized Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) Cholera enterotoxin subunit B, His Tag (Cat. No. GHB-V52H3) at 1 μg/mL (100 μL/well) can bind Anti-Cholera toxin, B Subunit (CTxB) Antibody with a linear range of 0.1-2 ng/mL (QC tested).

  • Background
    Cholera toxin is protein complex secreted by the bacterium Vibrio cholerae. It is responsible for the massive, watery diarrhea characteristic of cholera infection. The B subunit of cholera toxin (CtxB) binds to a GM1-ganglioside receptor, a ubiquitous glycolipid cell surface receptor. This binding is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. The beta chain has no toxic activity by itself. The holotoxin consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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