rh ADAM8 fused with a polyhistidine tag at the C-terminus, has a calculated MW of 71.6 kDa, which migrates as a 120-150 kDa band in DTT-reduced SDS-PAGE due to glycosylation. Two main fragments bands are visible with apparent MW of 67 kDa and 46 kDa respectively in reduced SDS-PAGE as a result of its being prone to proteolytic cleavage.
>85% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 20 mM CaCl2, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Human ADAM8, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
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