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Your Position: Home > Protein > Rnase Inhibitor > RNR-M5143

Murine RNase Inhibitor (40 U/μl)

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  • Product Details
    This product is a purified recombinant murine RNase inhibitor expressed in Escherichia coli with a molecular weight of approximately 50 kDa. The inhibitor can extensively inhibit RNase A, B, and C activity, and it inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. Compared with human RNase inhibitor, murine RNase inhibitor has significant antioxidant properties and is more suitable for reactions where low DTT concentrations are required (e.g., RT-PCR).
  • Application
    1. cDNA synthesis
    2. In vitro transcription/translation
    3. RT-PCR
    4. Enzymatic RNA labeling reaction
    5. Other applications where the integrity of RNA is important
  • Unit Definition
    One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.
  • Quility Control
    No endodeoxyribonucleases, exodeoxyribonucleases and Ribonucleases residues.
  • Purity
    >95% as determined by SDS-PAGE.
  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM HEPES 50 mM NaCl 50% Glycerol,8 mM DTT pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with blue ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. This product is stable for up to 12 months at -20℃ from date of receipt.
  • Notes

    The maintenance of the active state of this inhibitor requires at least 1mM DTT;

    This inhibitor does not inhibit the activity of RNase H;

    The inhibition of RNase activity has a wide pH range (pH 5-9), and shows maximum activity at pH 7-8.

    Avoid foaming, stirring vigorously, and vortexing to prevent inactivation of this product.

  • Clinical and Translational Updates

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