Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Kits > Th1/Th2 cytokine > FCM-C05R

ClinMax™ Human Th1/Th2 Cytokine Kit (Flow Cytometry Multiplex Bead Assay)

For research use only.

Order Now

Product Details
Assay Type FACS
Analyte IFN-γ
Format 96T
Reactivity Human
Regulatory Status RUO
Sensitivity 1.77pg/ml
Standard Curve Range 9.8~ 10000pg/mL
9.8~ 10000pg/mL
9.8~ 10000pg/mL
9.8~ 10000pg/mL
9.8~ 10000pg/mL
9.8~ 10000pg/mL
Assay Time 3 hr 8 min
Suitable Sample Type human serum, human plasma,cell culture supernatants
Sample volume 10 uL
Materials Provided
ID Components Size
FCM05-C01 Calibrator 5ug(for each)×2
FCM05-C02A Human IL-2 Capture Beads 96 tests
FCM05-C02B Human IL-4 Capture Beads 96 tests
FCM05-C02C Human IL-6 Capture Beads 96 tests
FCM05-C02D Human IL-10 Capture Beads 96 tests
FCM05-C02E Human TNF-α Capture Beads 96 tests
FCM05-C02F Human IFN-γ Capture Beads 96 tests
FCM05-C03 2 × Assay Buffer 40 mL
FCM05-C04 10 × Wash Buffer 10 mL
FCM05-C05 Detection antibody 0.5 mL
FCM05-C06 96-well V-bottom plate 1 plate
FCM05-C07 96-well Sealing film 2 pieces
FCM05-C08 APC Positive Control 0.5 mL
FCM05-C09 PE Positive Control 0.5 mL
  • Background
    T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After Th actived, it will differentiate into T-helper 1 (Th1) or T-helper 2 (Th2) effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized-some cytokines are more effective than others in the process of eliminating certain invaders. In general, TH1 cytokines play the role of immune killing, while Th2 cytokines play the role of immune protection. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.
  • Application

    The Human Th1/Th2 Cytokine Kit can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-6 (IL-6), Interleukin-10 (IL-10), Tumor Necrosis Factor-α (TNF-α), and Interferon-γ (IFN-γ) protein levels in a single sample.The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples.

    It is for research use only.

  • Storage
    Unopened kit should be stored at 2°C -8°C upon receiving.

    Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    Human Th1/Th2 Cytokine 6-plex panel is a  multiplex bead-based indirect immunofluorescence assay, utilizing flow cytometry for data  acquisition and classification. The beads conjugated with specific antibody, target protein (IL-2/4/6/10, TNF-α, IFN-γ) in specimens and PE-conjugated detection antibody form a sandwiches complex as bead-analyte-detection antibody. The intensity of PE fluorescence signal in proportion to the target protein  in specimens, are assessed by flow cytometry.The present assay has been optimized for quntitatively analysis for the IL-2/4/6/10, TNF-α, IFN-γ in cell culture supernatant, plasma, and serum samples.
Bioactivity-FACS Please refer to DS document for the assay protocol.
 Th1/Th2 cytokine FACS

A representative scatter plot showing single bead population in P1 gate (left), the beads conjugated with analyte-sepcific antibody differntiated by APC  fluorescence intensity (middle), and fluorescence signal in propotion to  concentration of  the analyte was measured for each cytokine in PE channel (right). Data was generated on Beckman Cytoflex S Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).

 Th1/Th2 cytokine FACS

A double logarithmic fitting curve was plotted for each cytokine( IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ). The present curve of each cytokines   was ranging from 9.8 pg/ml to 10000 pg/ml respectively .

  • Clinical and Translational Updates

Comments (0)

ETA of in-stock products: 2 business days

Price(USD) : $1500.00

Promotion & Exhibitions

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
+1 888-377-6111
1 Innovation Way, Newark, DE 19711, USA

Leave a message