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This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 68 kDa. The protein migrates as 75-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IGF-II R Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Human IGF-II, Fc Tag (Cat. No. IG2-H4260) at 5 μg/mL (100 μL/well) can bind Human IGF-II R Protein, His Tag (Cat. No. IGR-H52H3) with a linear range of 0.010-0.313 μg/mL (Routinely tested).
Loaded Human IGF-II R Protein, His Tag (Cat. No. IGR-H52H3) on HIS1K Biosensor, can bind Human IGF-II, Fc Tag (Cat. No. IG2-H4260) with an affinity constant of 15.6 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
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