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| ID | Components | Size |
| EP154-C01 | High-bind Plate | 1 plate |
| EP154-C02 | Human PD-1 | 15 μg |
| EP154-C03 | Biotinylated Human PD-L2 | 20 μg |
| EP154-C04 | Anti-PD1 Neutralizing Antibody | 20 μg |
| EP154-C05 | Streptavidin-HRP | 5 μg |
| EP154-C06 | Coating Buffer | 12 mL |
| EP154-C07 | 10xWashing Buffer | 50 mL |
| EP154-C08 | Blocking Buffer | 50 mL |
| EP154-C09 | Substrate Solution | 12 mL |
| EP154-C10 | Stop Solution | 7 mL |
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
INHIBITION OF PD-1: PD-L2 [Biotinylated] BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-PD-1 Neutralizing antibody (Catalog # EP154-C04) (1:1 serial dilution, from 5 μg/mL to 0.0098μg/mL) was added into PD-1: Biotinylated PD-L2 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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