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Your Position: Home > Protein > Ribonuclease 3 > RI3-E5143

RNase Ⅲ (2U/μl)

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  • Product Details
    RNase III enzyme typically cleave both strands of double-stranded RNAs (dsRNAs). This enzyme could convert long double-stranded RNA into a heterogeneous mix of short (18–25 bp) interfering RNAs (siRNA) suitable for RNA interference in mammalian cells.
  • Application
    1. Generate siRNA
    2. Gene silencing
    3. Remove dsRNA
  • Unit Definition
    One unit is defined as the amount of enzyme required to digest 1 µg of 500bp dsRNA to 12-30bp siRNA in 60 minutes at 37°C.
  • Quility Control
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 10 mM Tris, 500 mM NaCl, 0.5 mM EDTA, pH8.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with blue ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt.
    2. -20°C for 6 months under sterile conditions.
SDS-PAGE
Ribonuclease 3 SDS-PAGE

RNase Ⅲ (2U/μl) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Ribonuclease 3 MALS images

The purity of RNase Ⅲ (2U/μl) (Cat. No. RI3-E5143) is more than 90% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.

  • Clinical and Translational Updates

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