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| ID | Components | Size |
| RAP165-C01 | HBcAg Capture Antibody | 100 μg |
| RAP165-C02 | Biotinylated HBcAg Detection Antibody | 100 μg |
This pair is developed for detection of HBcAg in samples.
It is for research use only.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
● -20°C to -70°C for 36 months in lyophilized state;
● -70°C for 3 months under sterile conditions after reconstitution.
Your experiment will include 5 simple steps:
a) Coat the plate with HBcAg Capture Antibody.
b) Add your molecule of interest to the tests.
c) Add Biotinylated HBcAg Detection Antibody.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis.
Immobilized HBcAg Capture Antibody (Cat. No. RAP165-C01) at 1.0 μg/mL (100 μL/well) can bind HBcAg, and then add Biotinylated HBcAg Detection Antibody (Cat. No. RAP165-C02) at 1.0 μg/mL (100 μL/well). Detection was performed using HRP-conjugated streptavidin with a linear range of 3.9-125 ng/mL (QC tested).
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