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| Assay Type | Sandwich-ELISA |
| Analyte | T7 RNA polymerase |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | <0.78 ng/Ml |
| Standard Curve Range | 0.78 ng/mL-50 ng/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the quantitative determination of T7 RNA polymerase in Cell Culture Supernatants. |
| Sample volume | 100 uL |
| ID | Components | Size |
| RES018-C01 | Pre-coated Anti-T7 RNA polymerase Antibody Microplate | 1 plate |
| RES018-C02 | T7 RNA polymerase Standard | 100 μL |
| RES018-C03 | Biotin-Anti-T7 RNA polymerase Antibody | 150 μL |
| RES018-C04 | Streptavidin-HRP | 50 μL |
| RES018-C05 | 20xWashing Buffer | 50 mL |
| RES018-C06 | Biotin-Antibody and Streptavidin-HRP Dilution Buffer | 50 mL |
| RES018-C07 | Standard and Sample Dilution Buffer (5x) | 30 mL |
| RES018-C08 | Substrate Solution | 12 mL |
| RES018-C09 | Stop Solution | 7 mL |
T7 RNA Polymerase ELISA Kit (Residue Testing)was developed for the detection and quantitative determination of T7 RNA Polymerase in mRNA preparation processing where T7 RNA Polymerase is used as an row agent.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the T7 RNA polymerase as standard. The samples and standard are diluted by Dilution Buffer.
c) Add the Biotin-Anti-T7 RNA polymerase Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound enzyme.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜10%.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.
Three T7 RNA polymerases with different concentrations were tested to calculate the recovery rate.
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