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HEK293/Human TL1A Stable Cell Line

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  • Description
    The HEK293/Human TL1A Stable Cell Line was engineered to express the receptor full length human TL1A (Gene ID:9966). Surface expression of human TL1A was confirmed by flow cytometry.
  • Application

    • Useful for cell-based TL1A binding assay

  • Growth Properties
    Adherent
  • Selection Marker
    Puromycin (2 μg/mL)
  • Culture Medium
    DMEM + 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Bioactivity
 TL1A FACS

Expression analysis of human TL1A on HEK293/Human TL1A Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human TL1A Stable Cell Line or negative control cell using anti-human TL1A Antibody followed by staining with PE anti-human IgG Fc Antibody.

Passage Stability
 TL1A PASSAGE

Passage stability analysis of receptors expression by FACS.
Flow cytometry surface staining of human TL1A on HEK293/Human TL1A Stable Cell Line demonstrates consistent mean fluorescent intensity across across passage 5-24.

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  • Background
    TNF-like cytokine 1A (TL1A) and its receptors, death receptor 3 (DR3) and decoy receptor 3 (DcR3) are members of the TNF and TNF receptor superfamilies of proteins, respectively. Binding of APC-derived TL1A to lymphocytic DR3 provides co-stimulatory signals for activated lymphocytes. DR3 signaling affects not only the proliferative activity of and cytokine production by effector lymphocytes, but also critically influences the development and suppressive function of regulatory T-cells. Whereas, DcR3 restricts the function of the TL1A/DR3 complex: attenuating T-cell activation and downregulating the secretion of pro-inflammatory cytokines. Together with DR3 and DcR3, TL1A constitutes a cytokine system that actively interferes with the regulation of immune responses.
  • License Disclosure
    This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $3500.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:4 Details
  • Latest Research Phase:Phase 3 Clinical

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