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Your Position: Home > Kits > Siglec-2 > CEA-B046

ClinMax™ Human Soluble Siglec-2 / CD22 ELISA Kit, PRO

For research use only.

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Product Details
Assay TypeSandwich-ELISA
AnalyteSiglec-2/CD22
Format96T
ReactivityHuman
Regulatory StatusPRO
Sensitivity<35.0 pg/mL
Standard Curve Range93.75 pg/mL-6000 pg/mL
Assay Time3hr
Suitable Sample TypeFor the quantitative determination of human Soluble Siglec-2/CD22 in Cell Culture Supernatants, Plasma, Serum.
Sample volume50 μL
Materials Provided
IDComponentsSize
CEA046-C01Pre-coated Anti-Siglec-2/CD22 Antibody Microplate1 plate
CEA046-C02Human Siglec-2/CD22 Standard100 μg×2
CEA046-C03Biotin-Anti-Siglec-2/CD22 Antibody Con. Solution300 μL
CEA046-C04Biotin-Antibody Dilution Buffer8mL
CEA046-C05Streptavidin-HRP Con. Solution500 μL
CEA046-C06Streptavidin-HRP Dilution Buffer15 mL
CEA046-C0720× Washing Buffer50 mL
CEA046-C08Sample Dilution Buffer15 mL ×2
CEA046-C09Substrate Solution12 mL
CEA046-C10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human Siglec-2/CD22 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of human soluble Siglec-2/CD22 in serum, plasma and cell culture supernates.

    It is for research use only.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     Siglec-2 Assay Principles
Typical Data Please refer to Ds document for the assay protocol.
 Siglec-2 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of CD22 is less than 35 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 4 samples containing different CD22 concentrations were tested in one assay. Acceptable criteria: CV<10%.

 Siglec-2 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Four samples containing different concentrations of CD22 were tested in independent assays. Acceptable criteria: CV<15%.

 Siglec-2 INTER-ASSAY STATISTICS
Recovery

Recombinant CD22 was spiked into 3 human serum samples, and then analyzed. The average recovery of CD22 for serum samples is 105%.

 Siglec-2 RECOVERY
  • Background: Siglec-2
    CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases.
  • Clinical and Translational Updates

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