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| Assay Type | Sandwich-ELISA |
| Analyte | IL-12/IL-23 p40 |
| Format | 96T |
| Reactivity | Human |
| Sensitivity | <15.625 pg/mL |
| Standard Curve Range | 31.25 pg/mL-2000 pg/ml |
| Assay Time | 2hr |
| Suitable Sample Type | For the quantitative determination of human IL-12/IL-23 p40 in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 μL |
| ID | Components | Size |
| CEA203-C01 | Pre-coated Anti- IL-12/IL-23 p40 Antibody Microplate | 1 plate |
| CEA203-C02 | Human IL-12/IL-23 p40 Standard | 20μg ×2 |
| CEA203-C03 | Biotin-Anti-IL-12/IL-23 p40 Antibody Con. Solution | 100 μL |
| CEA203-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA203-C05 | Streptavidin-HRP Con. Solution | 500 uL |
| CEA203-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA203-C07 | 20× Washing Buffer | 50 mL |
| CEA203-C08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA203-C09 | Substrate Solution | 12 mL |
| CEA203-C10 | Stop Solution | 6 mL |
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of IL-12/IL23 p40 in human serum and cell culture supernates.
It is for research use only.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-12/IL-23 p40 is less than 15.625 pg/mL.
Ten replicates of each of 4 samples containing different IL-12/IL-23 p40 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Five samples containing different concentrations of IL-12/IL-23 p40 were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant IL-12/IL-23 p40 was spiked into 3 human serum samples, and then analyzed. The average recovery of IL-12/IL-23 p40 for serum samples is 103.2%.
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