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Your Position: Home > Cell > GLP1R > CHEK-ATP160

HEK293/Human GLP-1R Stable Cell Line (High Expression)

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  • Description
    The HEK293/Human GLP-1R Stable Cell Line was engineered to express the receptor full length human GLP-1R (Gene ID: 2740), with different levels of GLP-1R expression (High, Medium, Low). Surface expression of human GLP-1R was confirmed by flow cytometry.
  • Application

    • Useful for cell-based GLP-1R binding assay

    • Screen for human GLP-1R agonists based on cAMP accumulation assay

  • Growth Properties
    Adherent
  • Selection Marker
    Hygromycin (20 μg/mL)
  • Culture Medium
    DMEM + 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Bioactivity
 GLP1R FACS

Expression analysis of human GLP-1R on HEK293/Human GLP-1R Stable Cell Line by FACS.
Cell surface staining using PE-labeled anti-human GLP-1R antibody was performed on HEK293/Human GLP-1R Stable Cell Line with different expression levels: HEK293/Human GLP-1R Stable Cell Line (Low Expression); HEK293/Human GLP-1R Stable Cell Line (Medium Expression); HEK293/Human GLP-1R Stable Cell Line (High Expression).

Passage Stability
 GLP1R PASSAGE

Passage stability analysis of receptors expression by FACS.
Flow cytometry surface staining of human GLP-1R on HEK293/Human GLP-1R Stable Cell Line (High Expression) demonstrates consistent mean fluorescent intensity across passage 7-22.

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  • Background
    This gene encodes a 7-transmembrane protein that functions as a receptor for glucagon-like peptide 1 (GLP-1) hormone, which stimulates glucose-induced insulin secretion. This receptor, which functions at the cell surface, becomes internalized in response to GLP-1 and GLP-1 analogs, and it plays an important role in the signaling cascades leading to insulin secretion. It also displays neuroprotective effects in animal models. Polymorphisms in this gene are associated with diabetes. The protein is an important drug target for the treatment of type 2 diabetes and stroke.
  • License Disclosure
    This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $3500.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Phase 1 Clinical

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