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Your Position: Home > Protein > RNase H > RNH-E5113

RNase H (5U/μl)

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  • Product Details
    RNase H is an endoribonuclease that can specifically hydrolyze RNA in DNA-RNA hybrid chains. RNase H cannot digest single-stranded or double-stranded DNA or RNA.
  • Application
    1. Removal of mRNA during second-strand cDNA synthesis. Removal of poly(A) sequences from mRNA in the presence of oligo(dT). Oligodeoxyribonucleotide-directed cleavage of RNA.
  • Unit Definition
    One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 40 pmol of a fluorescently labeled 25 base pair RNA:DNA hybrid in a total reaction volume of 50 µl in 20 minutes at 37℃.
  • Quility Control
    No DNases and RNases residues.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 10 mM Tris, 0.1 mM EDTA, 1 mM DTT, 0.2% BSA, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with blue ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt.
    2. -20°C for 6 months under sterile conditions.
Bioactivity

One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 40 pmol of a fluorescently labeled 25 base pair RNA:DNA hybrid in a total reaction volume of 50 µl in 20 minutes at 37℃.

  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $45.00

Price(USD) : $157.00

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