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Your Position: Home > Immune Checkpoint Proteins > Human CD244 /2B4 /SLAMF4 Protein, With C-Fc Tag

Human 2B4 / CD244 / SLAMF4 Protein, Fc Tag

  • Synonym
    CD244,2B4,SLAMF4,NKR2B4,NAIL,h2B4
  • Source
    Human 2B4, Fc Tag (2B4-H5255) is expressed from human 293 cells (HEK293). It contains AA Cys 22 - Arg 221 (Accession # NP_057466).
    Predicted N-terminus: Cys 22
  • Molecular Characterization
    2B4(Cys 22 - Arg 221) NP_057466

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 48.9 kDa. As a result of glycosylation, the protein migrates as 65-80 kDa under reducing (R) condition, and 130-160 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human 2B4, Fc Tag (Cat. No. 2B4-H5255) SDS-PAGE gel

Human 2B4, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human 2B4, Fc TagHuman 2B4, Fc Tag (Cat. No. 2B4-H5255) ELISA bioactivity

Immobilized Human CD48, His Tag (Cat. No. BC1-H5226) at 5 μg/mL (100 μL/well) can bind Human 2B4, Fc Tag (Cat. No. 2B4-H5255) with a linear range of 0.313-5 μg/mL (QC tested).

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