2e5 of Anti-FAP CAR-293 cells were stained with 100 μL of 1 μg/mL FITC-Labeled Human FAP, Fc Tag (Cat. No. FAP-HF263) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested).
2e5 of anti-FAP CAR 293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human FAP, His Tag (Cat. No. FAP-HF243) and negative control protein respectively, FITC signals was used to evaluate the binding activity (QC tested).
Immobilized Monoclonal Anti-FAP (FAP5) Antibody, Mouse IgG2a (Cat. No. ) at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human FAP Protein, Fc Tag (Cat. No. FAP-HF263) with a linear range of 0.039-0.625 μg/mL (QC tested).
Immobilized Biotinylated Mouse FAP, His,Avitag (Cat. No. FAP-M82Q5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate. can bind Monoclonal Anti-FAP (FAP5) Antibody, Mouse IgG2a with a linear range of 0.3-2 ng/mL (QC tested).
FAP Molecule Synonym Name
FAP Molecule Background
FAP (also known as seprase) is a Type II transmembrane serine protease. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences. Degrade also gelatin, heat-denatured type I collagen. Also has dipeptidyl peptidase activity, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.