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PH20

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Cat. No. Species Product Description Structure Purity Feature
PH0-H5219 Human Human PH20 / SPAM1 Protein, Tag Free (MALS verified)
PH0-H5219-structure
PH0-H5219-sds
PH0-H5225 Human Human PH20 / SPAM1 Protein, His Tag (active enzyme) DMF Filed
PH0-H5225-structure
PH0-H5225-sds

Part of Bioactivity data

PH0-H5219-MALS-HPLC
Human PH20, Tag Free (Cat. No. ) MALS images

The purity of Human PH20, Tag Free (Cat. No. PH0-H5219) is more than 95% and the molecular weight of this protein is around 53-71 kDa verified by SEC-MALS.

Bioactivity

The activity of PH20 is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >55000 U/mg. (Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH5.35 at 37 °C in a 2.0 mL reaction mixture) (QC tested).

Synonym Name

SPAM1,PH-20,HYAL3,HYA1,HYAL1,HYAL5,SPAG15

Background

Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Clinical and Translational Updates

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