Loaded Human LRRC32, Fc Tag (Cat. No. LR2-H5256) on AHC Biosensor, can bind Human TGFB1, His Tag (Cat. No. TG1-H524x) with an affinity constant of 0.288 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Immobilized Human LRRC32, Fc Tag (Cat. No. LR2-H5256) at 2 μg/mL (100 μL/well) can bind Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (Cat. No. TG1-C5243) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells. LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells. The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation.