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Your Position: Home > MABSol® Biotin Labeled Proteins > Biotinylated Human PCSK9 Protein, C-His tagged Avi-tag

Biotinylated Human PCSK9, Avi Tag (Avitag™)

  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    MABSol® Biotinylated Human PCSK9 (PC9-H82E7) is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # AAI66619).
    Predicted N-terminus: Gln 31
  • Molecular Characterization
    PCSK9(Gln 31 - Gln 692) AAI66619

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 72 kDa. The protein migrates as 18 kDa and 66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and proteolytic digestion.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human PCSK9 (Cat. No. PC9-H82E7) SDS-PAGE gel

Biotinylated Human PCSK9 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity
Biotinylated Human PCSK9Biotinylated Human PCSK9 (Cat. No. PC9-H82E7) ELISA bioactivity

Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PCSK9, His Tag (Cat. No. PC9-H82E7) with a linear range of 0.03-0.5 μg/mL (QC tested).

Biotinylated Human PCSK9Biotinylated Human PCSK9 (Cat. No. PC9-H82E7) ELISA bioactivity

Immobilized Biotinylated Human PCSK9, His Tag (Cat. No. PC9-H82E7) can bind anti-Human PCSK9 Mab, Human IgG1 with a linear range of 0.05-1.5 ng/mL. The biotinylated PCSK9 is coated at 1 ug/mL (100 uL/well) on a streptavidin coated plates (Rountly tested).

  • Background
    Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Inventory : In stock

Price(USD) : $1380.00

Inventory : In stock

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