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Your Position: Home > Immune Checkpoint Proteins > B7-2 > Recombinant Cynomolgus B7-2 / CD86 / CD28 Ligand 2 Protein

Cynomolgus / Rhesus macaque B7-2 / CD86 Protein (HPLC-verified)

  • Synonym
    CD86,B7-2,B70,CD28LG2,LAB72,MGC34413
  • Source
    Cynomolgus/Rhesus macaque B7-2(HPLC-verified) (CD6-C52H5) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - His 240 (Accession # G7NXR4).
    Predicted N-terminus: Ala 19
  • Molecular Characterization
    B7-2(Ala 19 - His 240) G7NXR4

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.3 kDa. The protein migrates as 50-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus/Rhesus macaque B7-2(HPLC-verified) (Cat. No. CD6-C52H5) SDS-PAGE gel

Cynomolgus/Rhesus macaque B7-2(HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Cynomolgus/Rhesus macaque B7-2(HPLC-verified) (Cat. No. ) HPLC images

The purity of Cynomolgus / Rhesus macaque B7-2, His Tag (HPLC-verified) (Cat.# CD6-C52H5) was more than 95% as determined by SEC-HPLC.

  • Background
    Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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