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Your Position: Home > MABSol® Biotin Labeled Proteins > Mesothelin > Human MSLN /Mesothelin (aa 296-580) Protein, Biotinylated

Biotinylated Human Mesothelin / MSLN (296-580), ultra sensitivity (primary amine labeling)

  • Synonym
  • Source
    MABSol® Biotinylated Human Mesothelin / MSLN (296-580) (MSN-H8223) is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272). It is the biotinylated form of Human Mesothelin / MSLN (296-580) Protein (Cat # MSN-H5223).
    Predicted N-terminus: Glu 296
  • Molecular Characterization
    Mesothelin(Glu 296 - Gly 580) AAH09272

    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33 kDa. The protein migrates as 38-42 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 1.5-3.5 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human Mesothelin (aa 296-580) (Cat. No. MSN-H8223) SDS-PAGE gel

Biotinylated Human Mesothelin (aa 296-580) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Biotinylated Human Mesothelin (aa 296-580)Biotinylated Human Mesothelin (aa 296-580) (Cat. No. MSN-H8223) ELISA bioactivity

Immobilized Anti-MSLN MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human Mesothelin, His Tag (Cat. No. MSN-H8223) with a linear range of 0.031-0.25 μg/mL (QC tested).

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Price(USD) : $290.00

Price(USD) : $1250.00

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