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Your Position: Home > MABSol® Biotin Labeled Proteins > TFPI > Biotinylated Human TFPI Protein

Biotinylated Human TFPI, ultra sensitivity (primary amine labeling)

  • Synonym
    TFPI,LACI,TFPI1,EPI,TFI
  • Source
    MABSol® Biotinylated Human TFPI (TFI-H8226) is expressed from human HEK293 cells. It contains AA Asp 29 - Lys 282 (Accession # NP_006278.1). It is the biotinylated form of Human TFPI protein, His Tag (Cat # TFI-H5226).
    Predicted N-terminus: Asp 29
  • Molecular Characterization
    TFPI(Asp 29 - Lys 282) NP_006278.1

    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30 kDa. The protein migrates as 41-45 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 2-3 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human TFPI (Cat. No. TFI-H8226) SDS-PAGE gel

Biotinylated Human TFPI on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N­terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C­terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second K domain inhibits factor Xa; The third K domain binds to heparin; The C­terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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