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Your Position: Home > Immune Checkpoint Proteins > OX40 > Mouse TNFRSF4 /OX40/CD134 Protein

Mouse OX40 / TNFRSF4 / CD134 Protein

  • Synonym
    TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L
  • Source
    Mouse OX40, His Tag (OX0-M5228) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Pro 211 (Accession # NP_035789).
    Predicted N-terminus: Val 20
  • Molecular Characterization
    OX40(Val 20 - Pro 211) NP_035789

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 23.2 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse OX40, His Tag (Cat. No. OX0-M5228) SDS-PAGE gel

Mouse OX40, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Mouse OX40, His TagMouse OX40, His Tag (Cat. No. OX0-M5228) ELISA bioactivity

Immobilized Mouse OX40, His Tag (Cat. No. OX0-M5228) at 2 μg/mL (100 μl/well) can bind Mouse OX40 Ligand, Fc Tag (Cat. No. OXL-M526x) with a linear range of 2-30 ng/mL (QC tested).

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