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Your Position: Home > Immune Checkpoint Proteins > CD28 > CD8-M52H6

Mouse CD28 Protein, His Tag

  • Synonym
    CD28,Tp44
  • Source
    Mouse CD28, His Tag (CD8-M52H6) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Lys 149 (Accession # P31041-1).
    Predicted N-terminus: Asn 20
  • Molecular Characterization
    Online(Asn 20 - Lys 149) P31041-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.9 kDa. The protein migrates as 30-35 kDa and 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse CD28, His Tag (Cat. No. CD8-M52H6) SDS-PAGE gel

Mouse CD28, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Mouse CD28, His TagMouse CD28, His Tag (Cat. No. CD8-M52H6) ELISA bioactivity

Immobilized Mouse CD28, His Tag (Cat. No. CD8-M52H6) at 5 μg/mL (100 μL/well) can bind Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) with a linear range of 0.156-5 μg/mL (QC tested).

  • Background
    T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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