This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 19.2 kDa. The protein migrates as 21 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Biotinylated Human TNF-alpha, His Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human TNF-alpha, His Tag (HPLC-verified) (Cat. No. TNA-H82E3) was greater than 95% as determined by SEC-HPLC.
Recombinant Human TNF-alpha (Cat.No. TNA-H82E3) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.029-0.052ng/ml (Routinely tested).
Neutralization assay shows that the cytotoxicity effect of TNF-alpha (Cat.No. TNA-H82E3) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1ng/mL. The IC50 is 7ng/mL (Routinely tested).
Authors: Bourquard T, et al.
Journal: J Immunol 43388
Application: Homogeneous time-resolved fluorescence–based competition assay
Authors: Robert Karlsson, et al.
Journal: J Pharmaceut Biomed 2017
Authors: Ji Y, et al.
Journal: J Cell Biochem 2017
Application: Precipitation of TNFR Complexes
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Price(USD) : $350.00
Price(USD) : $1380.00
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