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Your Position: Home > Immune Checkpoint Proteins > PD-L1 > Biotinylated Human PD-L1 / B7-H1 Protein, Avitag,His Tag (recommended for biopanning)

Biotinylated Human PD-L1 / B7-H1 Protein, Avitag™,His Tag (recommended for biopanning)

  • Synonym
    PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1
  • Source
    Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) (PD1-H82E5) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).
    Predicted N-terminus: Phe 19
  • Molecular Characterization
    Online(Phe 19 - Arg 238) NP_054862.1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 27.8 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Application
    PD1-H82E5 works best for experiments that test the binding between PD-L1 and candidate antibodies, such as biopanning and other relevant assays.
    This product is NOT suitable for testing PD1-PDL1 binding. For this type of application, we strongly recommend you to choose PD1-H82F3 as an alternative.
  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E5) SDS-PAGE gel

Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning)Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E5) ELISA bioactivity

Immobilized Anti-Human PD-L1 MAb (Human IgG1) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E5) with a linear range of 0.05-2 ng/mL (QC tested).

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Price(USD) : $385.00

Price(USD) : $1518.00

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