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Your Position: Home > Bio-Markers & CD Antigens > IGFBP-4 > Human IGFBP-4 Protein

Human IGFBP-4 Protein

  • Synonym
    IGFBP4,IBP-4
  • Source
    Human IGFBP-4, His Tag (IG4-H52E4) is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Glu 258 (Accession # AAH16041).
    Predicted N-terminus: Asp 22
  • Molecular Characterization
    IGFBP-4(Asp 22 - Glu 258) AAH16041

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.9 kDa. The protein migrates as 33-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IGFBP-4, His Tag (Cat. No. IG4-H52E4) SDS-PAGE gel

Human IGFBP-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human IGFBP-4, His TagHuman IGFBP-4, His Tag (Cat. No. IG4-H52E4) ELISA bioactivity

Immobilized Human IGFBP-4, His Tag (Cat. No. IG4-H52E4) at 2 μg/mL (100 µl/well),can bind Human IGF-I, Fc Tag (Cat. No. IG1-H4269) with a linear range of 4-30 ng/mL (QC tested).

  • Background
    Insulin-like growth factor-binding protein 4 (IGFBP-4) is also known as IGF-binding protein 4, which contains an IGFBP domain and a thyroglobulin type-I domain. IGFBP-4 binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. IGFBP-4 is secreted by all colon cancer cells.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $2200.00

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