>ActiveMax® Recombinant Human VEGF110 (HPLC-verified)
This protein carries no "tag".
The protein has a calculated MW of 12.6 kDa. As a result of glycosylation, the protein migrates as 18-20 kDa under reducing (R) condition, and 40-45 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).
This product does not contain a heparin binding domain, and therefore doesn't bind with heparin.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
ActiveMax® Human VEGF110 on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of ActiveMax® Human VEGF110 (Cat.# VE0-H5212) was greater than 95% as determined by SEC-HPLC.
Immobilized Human VEGF110, Tag Free (Cat. No. VE0-H5212) at 2 μg/mL (100 μL/well) can bind VEGFR1/R2-Fc with a linear range of 1-10 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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