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Your Position: Home > Immune Checkpoint Proteins > CD40 > Biotinylated Human CD40 / TNFRSF5 Protein, Avitag,His Tag

Biotinylated Human CD40 / TNFRSF5 Protein, Avitag™,His Tag

  • Synonym
    CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50
  • Source
    Biotinylated Human CD40, Avitag,His Tag (CD0-H82E8) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # AAH12419.1).
    Predicted N-terminus: Glu 21
  • Molecular Characterization
    Online(Glu 21 - Arg 193) AAH12419.1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 21.8 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD40, Avitag,His Tag (Cat. No. CD0-H82E8) SDS-PAGE gel

Biotinylated Human CD40, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that Biotinylated Human CD40, Avitag,His Tag (Cat. No. CD0-H82E8) can bind to 293T cells overexpressing human CD40L. The concentration of Biotinylated Human CD40 is 3 μg/mL (Routinely tested).

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