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Your Position: Home > All Other Proteins > BCMA > Human BCMA / TNFRSF17 / CD269 Protein, His Tag

Human BCMA / TNFRSF17 Protein,His Tag

  • Synonym
    TNFRSF17,CD269,BCM,BCMA
  • Source
    Human BCMA, His Tag (BCA-H522y) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    BCMA(Met 1 - Ala 54) Q02223-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 7.8 kDa. The protein migrates as 12 kDa and 15-17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human BCMA, His Tag (Cat. No. BCA-H522y) SDS-PAGE gel

Human BCMA, His Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human BCMA, His TagHuman BCMA, His Tag (Cat. No. BCA-H522y) ELISA bioactivity

Immobilized Human BAFF, Fc Tag (Cat. No. BAF-H4268) at 5 μg/mL (100 μL/well) can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with a linear range of 4-125 ng/mL (QC tested).

Human BCMA, His TagHuman BCMA, His Tag (Cat. No. BCA-H522y) ELISA bioactivity

Immobilized Human BCMA, His Tag (Cat. No. BCA-H522y) at 2 μg/mL (100 μL/well) can bind Anti-Human BCMA Mab with a linear range of 0.2-1.6 ng/mL (Routinely tested).

Evaluation of CAR expression
FACS Analysis of Anti-BCMA CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were first stained with B. human BCMA / TNFRSF17 protein, His tag (Cat. No. BCA-H522y, 0.03ug/ml) and C. His tag protein control, followed by FITC-conjugated anti-6xHis tag antibody. A. Non-transfected 293 cells and C. His tag protein control were used as negative control. RFP was used to evaluate CAR (anti-BCMA-scFv) expression and FITC was used to evaluate the binding activity of human BCMA / TNFRSF17 protein, His tag (Cat. No. BCA-H522y).

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