This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 26.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
ActiveMax® Human Dkk-1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of ActiveMax® Human Dkk-1 Protein, His Tag (Cat. No. DK1-H5221) is more than 90% and the molecular weight of this protein is around 30-46 kDa verified by SEC-MALS.
Immobilized Human Dkk-1, His Tag (Cat. No. DK1-H5221) at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. LR5-H5254) with a linear range of 0.01-0.156 μg/mL (QC tested).
ActiveMax® Human Dkk-1 Protein, His Tag (MALS verified)(Cat.No. DK1-H5221) inhibits Wnt induced TCF reporter activity in HEK293 cells. Human Dkk-1 inhibits a constant dose of 0.03 µg/mL of Wnt surrogate. The EC50 for this effect is 0.050 µg/mL.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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