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Your Position: Home > All Other Proteins > CD4 > Biotinylated Human CD4 / LEU3 Protein, Fc Tag, Avi Tag

Biotinylated Human CD4, Fc Tag & Avi Tag (Avitag™)

  • Synonym
    CD4,CD4mut,LEU3
  • Source
    Biotinylated Human CD4, Fc Tag (CD4-H82F3) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # P01730-1).
    Predicted N-terminus: Lys 26
  • Molecular Characterization
    Online(Lys 26 - Pro 396) P01730-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 69.7 kDa. As a result of glycosylation, the protein migrates as 80 kDa under reducing (R) condition, and 150 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD4, Fc Tag (Cat. No. CD4-H82F3) SDS-PAGE gel

Biotinylated Human CD4, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CD4, Fc TagBiotinylated Human CD4, Fc Tag (Cat. No. CD4-H82F3) ELISA bioactivity

Immobilized HIV GP120 (HIV), His Tag (Cat. No. GP5-V15224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD4, Fc Tag (Cat. No. CD4-H82F3)) with a linear range of 0.5-8 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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CD4 Products

  • Name:T-cell surface antigen CD4
  • Classification:Homo sapiens
  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:39 Details
  • Lastest Research Phase:Approved
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