This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.3 kDa. The protein migrates as 28-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 100 mM Acetic Acid, pH2.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Rhesus macaque PLGF on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Rhesus macaque VEGF R1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE1-R5257) at 5 μg/mL (100 μL/well) can bind Rhesus macaque PLGF (Cat. No. PGF-R52H0) with a linear range of 2-40 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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