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Your Position: Home > All Other Proteins > PLGF > Rhesus macaque PLGF / PGF Protein

Rhesus macaque PLGF / PGF Protein

  • Synonym
    PGF,PLGF,PlGF2,PlGF,PGFL,SHGC-10760
  • Source
    Rhesus macaque PLGF (PGF-R52H0) is expressed from human 293 cells (HEK293). It contains AA Leu 19 - Arg 170 (Accession # F7HB10-1).
    Predicted N-terminus: Leu 19
  • Molecular Characterization
    PLGF(Leu 19 - Arg 170) F7HB10-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 19.3 kDa. The protein migrates as 28-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 100 mM Acetic Acid, pH2.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Rhesus macaque PLGF (Cat. No. PGF-R52H0) SDS-PAGE gel

Rhesus macaque PLGF on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Rhesus macaque PLGFRhesus macaque PLGF (Cat. No. PGF-R52H0) ELISA bioactivity

Immobilized Rhesus macaque VEGF R1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE1-R5257) at 5 μg/mL (100 μL/well) can bind Rhesus macaque PLGF (Cat. No. PGF-R52H0) with a linear range of 2-40 ng/mL (QC tested).

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