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Your Position: Home > All Other Proteins > BTN3A2 > Biotinylated Human BTN3A2 Protein, His Tag, Avi Tag

Biotinylated Human BTN3A2 Protein, His Tag, Avi Tag (Avitag™)

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  • Synonym
    BT3.2, BT3.3, BTF3, BTF4
  • Source
    Biotinylated Human BTN3A2, His Tag, Avi Tag (BT2-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Trp 248 (Accession # P78410-1).
    Predicted N-terminus: Gln30
  • Molecular Characterization
    BTN3A2(Gln 30 - Trp 248) P78410-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 27.3 kDa.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Contact us for customized product form or formulation.

    Please see Certificate of Analysis for specific instructions.

  • Storage

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

  • Background
    Butyrophilin subfamily 3 member A2(BTN3A2/BT3.2) mRNA expression by tumoral cells was previously identified as a prognostic factor in a small cohort of high grade serous epithelial ovarian cancer (HG-EOC).  We find that the three BTN3A isoforms: BTN3A1, BTN3A2, and BTN3A3, have high structural homology to the B7 superfamily of proteins and exist as V-shaped homodimers in solution, associating through the membrane proximal C-type Ig domain.
  • References
    • Limited Use License

      The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

      Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $350.00

    Price(USD) : $1380.00

    Promotion & Exhibitions

    • PEGS Boston 2018
    • WPC Boston 2018

    Datasheet & Documentation

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