0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Immune Checkpoint Proteins > BTLA > Biotinylated Human BTLA (31-150) Protein, Fc Tag, Avi Tag

Biotinylated Human BTLA (31-150) Protein, Fc Tag, Avi Tag (Avitag™)

  • Synonym
    BTLA,CD272
  • Source
    Biotinylated Human BTLA (31-150), Fc Tag, Avi Tag (BTA-H82F3) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1).
    Predicted N-terminus: Lys 31
  • Molecular Characterization
    BTLA(Lys 31 - Ser 150) AAP44003.1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 42.5 kDa. The protein migrates as 55-66 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arg, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human BTLA (31-150), Fc Tag, Avi Tag (Cat. No. BTA-H82F3) SDS-PAGE gel

Biotinylated Human BTLA (31-150), Fc Tag, Avi Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Biotinylated Human BTLA (31-150), Fc Tag, Avi TagBiotinylated Human BTLA (31-150), Fc Tag, Avi Tag (Cat. No. BTA-H82F3) ELISA bioactivity

Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BTLA (31-150), Fc Tag, Avi Tag (Cat. No. BTA-H82F3) with a linear range of 0.02-0.625 μg/mL (QC tested).

Comments (0)

This web search service is supported by Google Inc.

totop