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Your Position: Home > Protein > Fc gamma RIII / CD16 > FC6-R52H6

Rhesus macaque Fc gamma RIII / CD16 Protein, His Tag (SPR & BLI & MALS verified)

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  • Synonym
    FCGR3
  • Source
    Rhesus macaque CD16, His Tag (FC6-R52H6) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 206 (Accession # A3RFZ7-1).
    Predicted N-terminus: Glu 21
  • Molecular Characterization
    Online(Glu 21 - Gly 206) A3RFZ7-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 23.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rhesus macaque CD16, His Tag (Cat. No. FC6-R52H6) SDS-PAGE gel

Rhesus macaque CD16, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Rhesus macaque CD16, His Tag (Cat. No. ) MALS images

The purity of Rhesus macaque CD16, His Tag (Cat. No. FC6-R52H6) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Rhesus macaque CD16, His Tag (Cat. No. FC6-R52H6) captured on CM5 Chip via anti-His antibody can bind Rituximab with an affinity constant of 0.232 μM as determined in SPR assay (Biacore T200) (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Rhesus macaque CD16, His Tag (Cat. No. FC6-R52H6) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 0.28 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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