This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 111.5 kDa. The protein migrates as 110-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human FAP, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human FAP, Fc Tag (Cat. No. FAP-H5263) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-FAP (FAP5) Antibody, Mouse IgG2a with a linear range of 0.2-2 ng/mL (QC tested).
Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC).The specific activity is >4000 pmol/min/µg (QC tested).
2e5 of Anti-FAP CAR-293 cells were stained with 100 μL of 1 μg/mL of Human FAP Protein, Fc Tag (Cat. No. FAP-H5263) and negative control protein respectively, washed and then followed by PE anti-human IgG Fc antibody and analyzed with FACS (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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