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Your Position: Home > All Other Proteins > FAP > FAP-H5263

Human FAP Protein, Fc Tag (active enzyme)

  • Synonym
    FAP,FAPalpha,SIMP,Seprase,APCE
  • Source
    Human FAP, Fc Tag (FAP-H5263) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).
    Predicted N-terminus: Pro
  • Molecular Characterization
    Online(Leu 26 - Asp 760) Q12884-1

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 111.5 kDa. The protein migrates as 110-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human FAP, Fc Tag (Cat. No. FAP-H5263) SDS-PAGE gel

Human FAP, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human FAP, Fc TagHuman FAP, Fc Tag (Cat. No. FAP-H5263) ELISA bioactivity

Immobilized Human FAP, Fc Tag (Cat. No. FAP-H5263) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-FAP (FAP5) Antibody, Mouse IgG2a with a linear range of 0.2-2 ng/mL (QC tested).

Human FAP, Fc TagHuman FAP, Fc Tag (Cat. No. FAP-H5263) ELISA bioactivity

Immobilized Monoclonal Anti-FAP (FAP5) Antibody, Mouse IgG2a at 1 μg/mL (100 μL/well) can bind Human FAP, Fc Tag (Cat. No. FAP-H5263) with a linear range of 1-8 ng/mL (Routinely tested).

Human FAP, Fc TagHuman FAP, Fc Tag (Cat. No. FAP-H5263) ELISA bioactivity

Immobilized Human FAP, Fc Tag (Cat. No. FAP-H5263) at 1 μg/mL (100 μL/well) can bind Anti-FAP (Sibrotuzumab) Antibody,Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

Bioactivity

Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC).The specific activity is >4000 pmol/min/µg (QC tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-FAP CAR-293 cells were stained with 100 μL of 1 μg/mL of Human FAP Protein, Fc Tag (Cat. No. FAP-H5263) and negative control protein respectively, washed and then followed by PE anti-human IgG Fc antibody and analyzed with FACS (Routinely tested).

  • Background
    FAP (also known as seprase) is a Type II transmembrane serine protease. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences. Degrade also gelatin, heat-denatured type I collagen. Also has dipeptidyl peptidase activity, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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