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Your Position: Home > Cytokines > IL-5 > IL5-H82Q5

Biotinylated Human IL-5 Protein, His,Avitag™

  • Synonym
    IL-5,TRF,IL5,Interleukin-5
  • Source
    Biotinylated Human IL-5, His,Avitag (IL5-H82Q5) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Ser 134 (Accession # P05113-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Ile 20 - Ser 134) P05113-1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 16.7 kDa. The protein migrates as 20-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS,pH7.3. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-5, His,Avitag (Cat. No. IL5-H82Q5) SDS-PAGE gel

Biotinylated Human IL-5, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human IL-5, His,AvitagBiotinylated Human IL-5, His,Avitag (Cat. No. IL5-H82Q5) ELISA bioactivity

Immobilized Biotinylated Human IL-5, His,Avitag (Cat. No. IL5-H82Q5) at 1 μg/mL (100 μL/well) on Recombinant Streptavidin (Cat. No. STN-N5116 ) precoated (0.5 μg/well) plate, can bind Human IL-5 R alpha, Fc Tag (Cat. No. ILA-H5259) with a linear range of 5-78 ng/mL (QC tested).

  • Background
    Interleukin 5 (IL5) is an interleukin produced by type-2 T helper cells and mast cells. IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely interleukin 3 and GM-CSF), this glycoprotein in its active form is a homodimer. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma, wherein a large increase in the number of circulating, airway tissue, and induced sputum eosinophils have been observed. Given the high concordance of eosinophils and, in particular, allergic asthma pathology, it has been widely speculated that eosinophils have an important role in the pathology of this disease. Drugs that target IL-5 are mepolizumab and reslizumab.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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Drug Development Status

  • Number of Launched Drugs:2 Details
  • Number of Drugs in Clinical Trials:3 Details
  • Latest Research Phase:Approved

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