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Your Position: Home > Cytokines > IGF-I > IG1-M5253

Mouse IGF-I Protein, Fc Tag (MALS verified)

  • Synonym
    IGF-I,IGF1A,somatomedin C,MGF
  • Source
    Mouse IGF-I, Fc Tag (IG1-M5253) is expressed from human 293 cells (HEK293). It contains AA Gly 49 - Ala 118 (Accession # P05017-1).
    Predicted N-terminus: Pro
  • Molecular Characterization
    Online(Gly 49 - Ala 118) P05017-1

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 34.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse IGF-I, Fc Tag (Cat. No. IG1-M5253) SDS-PAGE gel

Mouse IGF-I, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Mouse IGF-I, Fc Tag (Cat. No. ) MALS images

The purity of Mouse IGF-I, Fc Tag (Cat. No. IG1-M5253) is more than 90% and the molecular weight of this protein is around 68-81kDa verified by SEC-MALS.

Bioactivity-ELISA
Mouse IGF-I, Fc TagMouse IGF-I, Fc Tag (Cat. No. IG1-M5253) ELISA bioactivity

Immobilized Mouse IGF-I R, His Tag (Cat. No. IGR-M5223) at 5 μg/mL (100 μL/well) can bind Mouse IGF-I Protein, Fc Tag (Cat. No. IG1-M5253) with a linear range of 0.02-1.25 μg/mL (QC tested).

  • Background
    Insulin-like growth factor 1 (IGF-1) is also known as somatomedin C, IGF1A, IGFI, sulfation factor, and is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. IGF-1 binds to at least two cell surface receptors: the Insulin-like growth factor 1 receptor, abbreviated as "IGF1R", and the insulin receptor. The IGF-1 receptor seems to be the "physiologic" receptor - it binds IGF-1 at significantly higher affinity than the IGF-1 that is bound to the insulin receptor. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. Its primary action is mediated by binding to its specific receptor IGF1R, present on many cell types in many tissues. Binding to the IGF1R, a receptor tyrosine kinase, initiates intracellular signaling; IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and proliferation, and a potent inhibitor of programmed cell death. Insulin-like growth factor 1 has been shown to bind and interact with all the IGF-1 Binding Proteins (IGFBPs), of which there are six (IGFBP1-6). Specific references are provided for interactions with IGFBP3, IGFBP4 and IGFBP7.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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